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Posted by Macabre
- October 01, 2013, 10:10:44 PMI ran across this article today:
http://onlinelibrary.wiley.com/doi/10.1111/cea.12207/abstract;jsessionid=2FA0A5C192D3EA3D4AF94E2D1DF2331A.f01t02
Identification of putative new tomato allergens and differential interaction with IgEs of tomato allergic subjects
Saskia Welter1,*, Karola Lehmann2, Sabine Dölle3, Dietmar Schwarz1, Wolfram Weckwerth4, Christian Scheler2, Margitta Worm3, Philipp Franken1
DOI: 10.1111/cea.12207
Abstract
Background
Tomato became one of the worldwide most consumed vegetables, unfortunately accompanied by an increasing risk for tomato allergy affecting certain people. Since tomato allergic subjects show highly variable reactions in clinical allergy tests, it is difficult to identify cultivars or differentially treated tomato plants where a significant reduction in the allergenic potential over all subjects of a cohort can be detected.
Objective
This study was carried out in order to test the hypothesis that individual variability is based on differential reactions of single subjects to particular allergens in tomato fruits of plants with certain genetic background or cultivated under distinct conditions.
Methods
Proteins were extracted from tomato fruits of the previously investigated genotypes 76R, its mycorrhizal mutant RMC, and the cultivar Counter, fertilised with different forms of nitrogen in deficit or excess. 2-D immunoblots were carried out with sera of nine tomato allergic subjects, beforehand analysed in skin prick tests.
Results
In total ten putative tomato allergens were identified in these immunoblots. No correlation was detected between individual skin prick test results and the quantity of positive reactions to putative allergens. IgEs of each subject showed reactions to nearly every identified putative allergen, but reactions were dependent on genotype and growth conditions. Among the ten putative tomato allergens five new candidates were identified: an endo-β-mannanase, a pectinacetylesterase, a pectinesterase inhibitor, an aspartyl protease family protein, and a protein of unknown function.
Conclusion and clinical relevance
The hypothesis that high inter-individual differences in allergic reactions are based on the interactions between the IgEs of allergic subjects with particular allergens has to be rejected. However, five proteins with putative clinical relevance as tomato allergens could be newly identified.
This article is protected by copyright. All rights reserved.
http://onlinelibrary.wiley.com/doi/10.1111/cea.12207/abstract;jsessionid=2FA0A5C192D3EA3D4AF94E2D1DF2331A.f01t02
Identification of putative new tomato allergens and differential interaction with IgEs of tomato allergic subjects
Saskia Welter1,*, Karola Lehmann2, Sabine Dölle3, Dietmar Schwarz1, Wolfram Weckwerth4, Christian Scheler2, Margitta Worm3, Philipp Franken1
DOI: 10.1111/cea.12207
Abstract
Background
Tomato became one of the worldwide most consumed vegetables, unfortunately accompanied by an increasing risk for tomato allergy affecting certain people. Since tomato allergic subjects show highly variable reactions in clinical allergy tests, it is difficult to identify cultivars or differentially treated tomato plants where a significant reduction in the allergenic potential over all subjects of a cohort can be detected.
Objective
This study was carried out in order to test the hypothesis that individual variability is based on differential reactions of single subjects to particular allergens in tomato fruits of plants with certain genetic background or cultivated under distinct conditions.
Methods
Proteins were extracted from tomato fruits of the previously investigated genotypes 76R, its mycorrhizal mutant RMC, and the cultivar Counter, fertilised with different forms of nitrogen in deficit or excess. 2-D immunoblots were carried out with sera of nine tomato allergic subjects, beforehand analysed in skin prick tests.
Results
In total ten putative tomato allergens were identified in these immunoblots. No correlation was detected between individual skin prick test results and the quantity of positive reactions to putative allergens. IgEs of each subject showed reactions to nearly every identified putative allergen, but reactions were dependent on genotype and growth conditions. Among the ten putative tomato allergens five new candidates were identified: an endo-β-mannanase, a pectinacetylesterase, a pectinesterase inhibitor, an aspartyl protease family protein, and a protein of unknown function.
Conclusion and clinical relevance
The hypothesis that high inter-individual differences in allergic reactions are based on the interactions between the IgEs of allergic subjects with particular allergens has to be rejected. However, five proteins with putative clinical relevance as tomato allergens could be newly identified.
This article is protected by copyright. All rights reserved.